Excel was used to plot the histogram based on the nucleotide distances. Pseudomonas strains with polyethylene degradation Archives of Pharmacy Practice ¦ Volume 10 ¦ Issue 4 ¦ October-December 20191 45 selected based on 1000 replications for the MEGA7 program. Plastic transparent was preferable degraded than black and white plastic bag `kresek'. Bacillus PL-01 put higher influent than Pseudomonas PL-01. Index Terms— M9 media, pseudomonas … H "Plastic (LDPE) Degradation by Induced Mutations in P seudomanas Putida ""IOSR Journal of Environmental Science, Toxicology and Food Technology (IOSR-JESTFT) 12.9 (2018): 34-40 This table shows 15 bacterial genera which have the capacity to degrade various types of plastics. This result is achieved due to addition of starch as additive in M9 media. This study reveals that pseudomonas sp. A number of microorganisms have the ability to degrade different types of plastic under suitable conditions, but due to the hardness of these polymers and their non-solubility in water, biological decomposition is a slow process. Plastic disposal is one of the greatest problems facing the environment today, as vast amounts of synthetic plastic remain non degradable.

But anyhow, indigenous mangrove soil bacteria showed the best performance in biofilm formation and plastic degradation, even without Pseudomonas PL-01 or Bacillus PL-01 addition. posses greater potential to degrade polyethylene. Plastic transparent was preferable degraded than black and white plastic bag ‘kresek’. Raziyafathima et al 235 J Pharm Chem Biol Sci, June-August 2016; 4(2):231-242 Aspergillus glaucus Polythene and plastic mangrove soil 20.80% and 7.26% [66] Micrococcus luteus Plastic cup Forest soil 38% [67] Masoniella sp Plastic cup Forest soil 27.4% [67] Pseudomonas sp and bacillus cereus Bacillus PL-01 put higher influent than Pseudomonas PL-01. There are some microorganisms that have the capacity to degrade plastic waste up to 51.5%. Among them, Pseudomonas is dominant. The degradation of most synthetic plastics in nature is a very slow process that involves environmental factors, followed by the action of wild microorganisms (Albertsson, 1980, Cruz-Pinto et al., 1994, Albertsson et al., 1994). It can degrade polythene, PVC, PHB, poly(3-hydroxybutyrate-co-3-mercaptopropionate), and poly(3-droxypropionate). But anyhow, indigenous mangrove soil bacteria showed the best performance in biofilm formation and plastic degradation, even without Pseudomonas PL-01 or Bacillus PL-01 addition.

Compared with the conventional film-type of plastic, the degradation efficiencies to the bead form of plastic were significantly improved and thus the biodegradation time was dramatically shortened.


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