EXERCISE 13
LABORATORY RESULTS WORKSHEET
Name Lab Coordinator
1. Autoimmune hemolytic anemia in children: Classification, clinical features, and diagnosis. Preferred Specimen: Gold Top (Serum Separator Tube) Acceptable: Red Top. Found inside – Page 846... platelet inhibition r Hydroxychloroquine: Retinotoxicity, cardiotoxicity, neurotoxicity DIAGNOSTIC TESTS & INTERPRETATION Labs/Studies r CBC to rule out anemia, thrombocytopenia, leukopenia. If anemic, consider hemolysis workup. r ... ; Reticulocyte count was normal 0.5 to 1.5%. so�z8w��˓E�r�\�f��tZ��aX�X��J:خ��Wk���t�Z����0i��!k4d�A�R���y��'WU6�����26� 2�\�߇���ZS�ֆ����j +�`wL7P�9�LҞZFKV!���6-7הci4w���U�b���d}��zR�Fװ�}�L��1J��Ǻ,)���v�U�V��cLv6�-Ȫ��3DzJ�q�D[��)����;B�"2�� ��6\��38���t)��]��{��\Y�˼�7r
�,Ov���+G�ܨxӎ. Hemolytic anemia (HA) is characterized by increased red cell destruction and a decreased red cell life span. hemolytic anemia include infection, certain medications, autoimmune Blood Agar and Hemolysis. �\}��h��Lz��E0K�ʦ�5�=��[o+�f�)`�����j��4C�QCt���!Dܛ|��7�:�Z4.F�����\�,W���EŰ^�h � ����u�t%�T��+�S�}�� 4. Each test is approached with the following format: . Found inside – Page 263A novel tube assay has become available using a red cell suspension (Shigeta Pharmaceutical,) but this test is not ... B cat (but also type B blood given to a type A cat) results in a very serious acute hemolytic transfusion reaction. As a result of a single point mutation in the beta-hemoglobin gene, the hemoglobin molecule of patients with sickle cell disease is less soluble under deoxygenated conditions. Found inside – Page 261... gene mutations in, 228-230 Hematopoietic neoplasia, molecular diagnosis of, 219 Hemolysis, 27, 32 defined, ... 162-163 interpretation of HLA test results, 164 HNPCC (hereditary nonpolyposis colon cancer), 150-152 Homoduplex, ... 2. Which factor(s) does S. aureus provide and how? � In a flowchart format, diagram your approach from receiving your Unknown through presumptive identification. Found inside – Page 478Labs taken at this visit revealed the following: WBC = 4.0 × 109/L, Hgb = 10g/dL, Hct = 30%, platelets = 230,000, ... can yield kinetic and morphologic laboratory results that are indistinguishable from those of hemolytic anemia. Interpretation. What is the MBC (if determined) of tetracycline for this isolate of S. aureus? 70-80 % of decisions in diagnosis are based on laboratory results and more and more laboratory analyses are requested. It is normally found within red blood cells (RBCs . Describe the gram-stained appearance and draw your microscopic observations of each of the organisms and their endospores. stream
Genetic testing is indicated when initial test results do not explain clinical presentation or mode of inheritance. Unknown # Antibiotic concentration
EXERCISE 8
LABORATORY RESULTS WORKSHEETS (2 pages)
Name Lab Coordinator
1. Gauge of needle, arm position, location of venipuncture, antiseptic used for The reference range does not vary with age, gender, race, or ethnicity. 0.75 g/dl sodium chloride solution (incubated) males: 0.5% to 8.5% hemolysis females: 0.1% to 9.3% hemolysis. Sickle cell anemia (SCA) is one of the most common genetic disorders. Phillips J, Henderson AC. Hemoglobin is a respiratory pigment that has an insatiable passion for oxygen and gloms onto every molecule it can hold while passing through the lungs, then dumps . In a flowchart format, diagram your approach from receiving your Unknown through presumptive identification. ��ࡱ� > �� � � ���� � � ���������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������� q` �� F� bjbjqPqP �� : : T/
�� �� �� � � � � � � � � � p �P �P �P � �Q T (R p �\ 8S 8S 8S 8S 8S 8S 8S 8S '\ )\ )\ )\ )\ )\ )\ $ �] h F` � M\ 9 � 8S 8S 8S 8S 8S M\ � � 8S 8S �\ DT DT DT 8S R � 8S � 8S '\ DT 8S '\ DT DT � s[ h � � �[ 8S ,S @,S��� �P �S R �[ \ �\ 0 �\ �[ �` �S �` �[ �` � �[ 8S 8S DT 8S 8S 8S 8S 8S M\ M\ �S R 8S 8S 8S �\ 8S 8S 8S 8S p p p �K �P p p p �P p p p � � � � � � ���� EXERCISE 1
LABORATORY RESULTS WORKSHEET
Name Lab Coordinator
1. 2. The sediment is resuspended in the remaining supernatant by flicking the bottom of the tube several times. The extracellular enzymes produced by these bacteria are called hemolysins which radially diffuse outwards from the colonies, causing complete or partial lysis of the red blood cells. These can be differentiated in most instances on the basis of history, clinical findings, and other laboratory results (RBC morphologic features, total protein, iron parameters . 2. depends upon the type and cause of the hemolytic anemia. This useful for the screening of occult alcoholism. Record the results of tests performed on each organism. Am J Clin Pathol 1997;107(3):353-8. Name Lab Coordinator . Given the widespread use of haptoglobin testing, it is vital that clinicians and laboratory staff understand the principles and correct interpretation of this test. Complete the following worksheet recording the size of each zone of inhibition:
Average Diameter
Amount of antibiotic of Zone of Inhibition
24 �g
12 �g
6 �g
3 �g
Record the zone diameter around the sample with unknown antibiotic concentration. Hemolysis is the destruction of red blood cells. Haptoglobin testing in the setting of inflammation is additionally discussed as interpretation can be difficult in this setting. Was it a penicillinase producer? Terms to Know: Describe the appearance of colonies and color of zones surrounding colonies present on mannitol salt media. Post a free question. Hemolysis releases hemoglobin into the blood stream. Name Lab Coordinator . Not Preferred: EDTA plasma (Lavender Top Tube), Green top (Lithium Heparin Plasma Separator Tube) Standard Volume. HAEV1 : Hemolytic anemia (HA) is characterized by increased red cell destruction and a decreased red cell life span. autoimmune hemolytic anemia, Non-immune Found inside – Page 438< 20 % of RBCs are stomacytic in many acquired disorders ( e.g. , alcoholism , druginduced hemolytic anemia , various neoplasms , hepatobiliary disease ) . Laboratory findings resemble those of hereditary spherocytosis with variable ... Blood contains inhibitors for certain bacteria such as Neisseria and Haemophilus genera and the blood agar must be heated to inactivate these inhibitors and to release essential growth factors (e.g., V factor). ‒hemolytic anemia, blood incompatibility, infection, other rare diseases Found inside – Page 186DESCRIPTION r A recognized complication of preeclampsia (PEC)-eclampsia that involves Hemolysis, Elevated Liver enzymes, ... papilledema/retinal hemorrhages possible DIAGNOSTIC TESTS & INTERPRETATION Lab Initial lab tests r All labs as ... weakness, paresthesia, hemolysis, platelet dysfunction and cardiac and respiratory failure. Explain what a positve agglutination test means. This disease may The tests that make up a lipid profile are tests that have been shown to be good indicators of whether someone is likely to have a heart attack or stroke caused by blockage of blood vessels (hardening of the arteries). Blood chemistry testing is defined simply as identifying the numerous chemical substances found in the blood. The Hemolysis test is designed to determine the hemolytic properties of a medical device materials that have direct or indirect blood contact and fulfills hematological testing requirements for selection of tests for interactions with blood. Record the microscopic appearance of cells from each culture. Lab Dept: Hematology Test Name: OSMOTIC FRAGILITY General Information Lab Order Codes: OSFM Synonyms: RBC Fragility; Erythrocyte Fragility; Incubated Osmotic Fragility CPT Codes: 85557 -Osmotic fragility, RBC; incubated Test Includes: Percent hemolysis of red blood cells for unincubated specimens placed in 0.50 g/dL concentration of NaCl and incubated specimens (37°C for 2. 2. Found inside – Page 257Prepare infant for laboratory test, for example: (1) Wrap foot for capillary specimen—a wrapped foot arterializes specimen and ... (use sodium heparin tube), ammonia levels, and Laboratory Concepts and Test Interpretation m CHAPTER 13 257. compensate, anemia does not occur. A regenerative anemia results from loss of RBC (hemorrhage) or destruction of RBC (hemolysis). Creatinine Serum creatinine can often be elevated as a result of acute kidney injury from hemoglobinuria from hemolysis and/or heart failure. Anemia testing usually takes place in a doctor's office, laboratory, or hospital. Anemia is a decrease in the number of red blood cells (RBCs—as measured by the red cell count, the hematocrit, or the red cell hemoglobin content). � Laboratory factors causing hemolysis are those related to collection, transport, processing etc. Montvale, NJ: Thomson PDR; 2007. The lipid profile includes total cholesterol , HDL-cholesterol (often called good cholesterol), LDL-cholesterol (often called bad cholesterol), and triglycerides . x��[mo�8� ��_ The Hemolysis test is designed to determine the hemolytic properties of a medical device/material. This book will help identify the areas where hemolysis occurs most frequently, which can, in turn, guide further analysis about why it is occurring. Complete the following table and identify each organism. � For example, testing may be divided into Tier One and Tier Two. Hemolysis is the lysis of red blood cells in the blood due to the extracellular enzymes produced by certain bacterial species. Found inside – Page 1107Virus induced hemolysis in equine infectious anemia . ... The detection of intravascular hemolysis in the horse . ... Al - 07474-03 ( BM ) LOZANO , EDGARDO A , Veterinary Res Lab , Montana State Univ , Bozeman , Mont . 59715. Complete the following table:
Organism Requires Requires Requires Both Satellite growth
Tested Hemolysis X only V only X and V with S. aureus
Identification of Unknown =
Identification of Unknown =
4. Useful For. One of the most commonly encountered interferences in clinical laboratories is hemolysis, and electrophoresis can be affected by this interference. %PDF-1.5
Found inside – Page 394... J. C. Summary of results of antenna design cost studies ( AD - 776914 ) 15 p1769 N 74-25769 SUREK , T. Continuous silicon ... D. E. Full - scale aircraft cabin flammability tests of improved fire - resistant materials INASA - TM - X ... indirect �X�wR.S��y�����'�9g��2�3>���k|��������2;��?��+\Ȧ_=��Xn�a�i%(DҴ���_0���k�gXo�ע��ۯ̤���{��V��|�%N0��8%?k#'�2�җ�m�p�2-|��Gz�pn3���IlfӢ�eiw������9*�Y��SN�J��ˋ���QUY�:z�ߒ��X��c�x Found inside – Page 31However, to accurately distinguish β-hemolysis with the naked eye in all possible manifestations is difficult even for a skilled microbiologist. This requires caution and experience and, especially under high labs load, it is an error ... count, Low For each organism, record the appearance of a colony on blood agar plates and draw/describe the zones of hemolysis seen surrounding the colonies. Definition of hemolytic anemia Definition of hemolytic anemia: This is a hemolytic state when the RBCs life in vivo is shortened. Found inside – Page 171Disaccharide Absorption Tests Normal: This test is used to diagnose malabsorption caused by disaccharide deficiency. ... Donath-Landsteiner (D-L) Test for Paroxysmal Cold Hemoglobinuria Normal: no hemolysis Interpretation: hemolysis ... Interpretation of the results for the evaluation of hemolytic anemia Evaluation of lifelong or inherited hemolytic anemias, including red cell membrane disorders, unstable or abnormal hemoglobin variants, and red cell enzyme disorders Laboratory Values and Interpretation of Results. Many laboratories report results and alert clinicians to interpret the results in the presence of . LABORATORY RESULTS WORKSHEETS (cont.) urine and fecal urobilinogen, Elevated Hemolytic anemia (Note: If any other size lavender tube is used, the tube must be filled to at least 50% capacity of tube fill volume.Insufficient volume may limit the extent of procedures performed.) � • Relate microbial structure to mode of anti-microbial action. Video chat with a U.S. board-certified doctor 24/7 in less than one minute for common issues such as: colds and coughs, stomach symptoms, bladder infections, rashes, and more. disease. Background: Preanalytical factors are the main source of variation in clinical chemistry testing and among the major determinants of preanalytical variability, sample hemolysis can exert a strong influence on result reliability. of red blood cells by increasing their production. for hemolysis (red blood cell destruction). Found inside – Page 556Indirect Hemolysis, Gilbert's syndrome, resorption of blood after hyperbilirubineElevated indirect>direct bilirubin □ mia □ trauma . Table 17.3 Interpretation of hepatitis A testing Anti-HAV Possible interpretation − − Anti- HAV IgG ... Mac- Gas
Organism Gram TSI MIO Agar . Normally, red blood cells have a lifespan of 120 days. How does your common microbiota isolate compare to the laboratory strain? � anemia due to G6PD deficiency, Idiopathic factor), or outside the red blood cell (extrinsic factor). PDR Lab Advisor. laboratory tests. decreased life span. females: 0.5% to 28.9% hemolysis. This test measures the amount of haptoglobin in the blood. This process can be something chronic that has occurred over time or acute and life-threatening. hemolysis. Hemolysis. Found inside – Page 291Pseudo-hyperphosphatemia: Conditions such as hyperglobulinemia, hyperlipidemia, hemolysis, high dose liposomal amphotericin ... Lab tests confirm an increase in serum creatinine. Urine output for the past 24 hours has been 800 mL. Labs ... Patients 2, 3, and 4 experienced severe hemolysis with 2 to 4 g/dL hemoglobin decrease. Complete the following table and identify each organism. The fourth edition of this leading text reflects the new direction and growth of the field of hematology as an academic and clinical discipline. Click on the link to view a sample search on this topic. Elevated Causes of Cryoglobulinemia, giant platelets, and white blood count > 100,000/microliter may cause a spurious high RBC. The purpose of this laboratory seminar is to discuss the interpretation of the test called CBC. What is the MIC of tetracycline for your fingertip isolate? Laboratory medicine is typically divided into three main phases (pre-analytical, analytical and post-analytical). Found insideHypogammaglobulinemia • In cases associated with AIHA, labs consistent with hemolysis may be present (elevated LDH, total bilirubin; reticulocyte count does ... Test Interpretation • A bone marrow aspirate usually shows >30% lymphocytes. • Moderate = 8-10 g/dL: Hemolysis = breaking of red cells hemolysis regardless of cause leads to anemia , low hemoglobin this is called anemia. Hemoglobin is the iron-containing protein complex that transports oxygen throughout the body. Found inside – Page 322150 A STUDY OF HEMOLYSIS BY ULTRASONICS ( Untersuchungen zur Klärung der ... 2151 TESTS SHOW AIRSICKNESS CAN BE STOPPED ; AMERICAN AVIATION 6 Aug '51 ( 15-10 Semi - Mthly ) ; pp 26- ; illus . 2155 CORROSION FACTORS IN DESIGN , F. M. ... Beta hemolysis (β) is defined as complete or true lysis of red blood cells. Haptoglobin testing is used primarily to help detect and evaluate hemolytic anemia and to distinguish it from anemia due to other causes. Specimens received in lab after 1:30pm will not be tested until the following day. 3. with your health care provider if symptoms of hemolytic anemia develop. So hemolysis is literally the destruction of blood cells, specifically red blood cells. Neonatal Laboratory Interpretation Linda J. Juretschke, PhD, RNC-NPT, APN/NNP-BC, CCNS Founder/President, NICU Solutions, Inc. Neonatal Nurse Practitioner The Ronald McDonald Children's Hospital at Loyola University Medical Center Objectives At the end of this lecture, the participant will be able to: 1. � Interpretation* of Hemolysis on Blood Agar Plates (*) To read the hemolytic reaction on a blood agar plate, the plate must be held up to a light source and observed with the light coming from behind (transmitted light). Laboratory abnormality Metabolism abnormality [ more] 0001939 . Found insideHypogammaglobulinemia In cases associated with AIHA, labs consistent with hemolysis may be present (elevated LDH, total bilirubin; reticulocyte count does not ... Test Interpretation A bone marrow aspirate usually shows >30% lymphocytes. Interpretation of the results for the evaluation of hemolytic anemia. Hemoglobin is a respiratory pigment that has an insatiable passion for oxygen and gloms onto every molecule it can hold while passing through the lungs, then dumps . EXERCISE 8
LABORATORY RESULTS WORKSHEETS (cont.) Organism =
Resistant,
Amount of Diameter of Intermediate,
Antibiotic Antibiotic Zone of Inhibition or Susceptible
Penicillin G
Ampicillin
Cephalothin
Erythromycin
Tetracycline
Methicillin
Streptomycin
EXERCISE 7
LABORATORY RESULTS WORKSHEETS (cont.) 2. all types of lab tests,Hematological tests,blood diseases test,diabetes profile,Lipid profile,Kidney profile,Muscle profile,Pancreas profile. Four of 6 patients reported fever. IV collection, capillary collection frequently cause hemolysis. Complete the following worksheet:
Acid from
Organism Colony Gram b�- l a c t a m a s e M a n n i t o l G l u c o s e T u b e
T e s t e d M o r p h o l o g y S t a i n H e m o l y s i s C a t a l a s e A c t i v i t y F e r m e n t a t i o n A n a e r o b i c a l l y C o a g u l a s e
Identification of Unknown = Identification of Unknown =
6. The results of the lipid profile are considered, Hemolytic anemia How is a lipid profile used? Patients usually have decreased hemoglobin concentration, hematocrit, and red blood cell count, but some can have compensated disorders, and symptoms such as reticulocytosis, pigmented gallstones, and decreased haptoglobin are factors that raise clinical suspicion. Found inside – Page 126enzymes, hemolysis, even slight, may strongly infiuence the plasma or serum enzyme activity. ... 2), the remoteness of the laboratory from sampling place and the carrying conditions to labs including shaking on rough roads can cause ... Found inside – Page 818... Additional information on Antithrombin. preanalytical, analytical (the test), and postanalytical (interpretation) phases. ... due to variation in interpretation associated with hematocrit, hemolysis, choice of anticoagulant, ... Clinical laboratory test results are a very important parameter in diagnosis monitoring and screening. Sucrose hemolysis test (confirmatory) Hemolysis findings in supernate are as follows: < 5% - Inconsequential or negative. 3. Conkey Brilliant Sugar Utilization . Hemolytic anemia is defined by the premature destruction of red blood cells, and can be chronic or life-threatening. This enzyme deficiency is a genetic disorder that affects mostly males. Record the degree of agglutination (0 to 4+) for each antigen:
Serum
Final Serum O Antigen H Antigen
Tube # Dilution Titer After 1 Hour 2nd Lab Period After 1 Hour 2nd Lab Period
1 1/5 5
2
3
4
5
6
7
8
9
10
2. Tested Proteolysis Observations Growth Production Reduction Production Lactose Sucrose Salicin
Identification of Unknown =
Identification of Unknown =
EXERCISE 9
LABORATORY RESULTS WORKSHEET
Name Lab Coordinator
1. View in Chinese. First Tier testing is designed to detect hemolysis, but cannot differentiate immune from other causes of hemolysis. Hemolytic anemia is defined as anemia due to a shortened survival of circulating red blood cells (RBCs) due to their premature destruction. Most vascular cell damage occurs during phlebotomy; this is the most frequent reason for specimen rejection. Found inside – Page 112... symptoms, and treatment similar to preeclampsia LABS • CBC • Liver function panel • Kidney function panel • PT/PTT • Fibrinogen • Urine dipstick/urinalysis • 24-hour urine collection LAB INTERPRETATION • Hemolysis • Increased liver ... Found inside – Page 78Albumin concentration at most veterinary labs is measured by the bromocrescol green dye (BCG)-binding reaction in ... In the presence of hemolysis sufficient to cause red plasma discoloration, the results of biuret total protein and BCG ... EXERCISE 8. Complete the following table:
Tests ' ( . On semi-log paper, plot the standard curve of antibiotic concentration versus the diameter of the inhibitory zone observed in 7.2.3. � What is the biochemical basis for the color change in the media? LAB8341. EXERCISE 8. &. Draw the reaction seen on egg yolk medium with each organism. types of hemolytic anemia, which are classified by the location of the defect. First ed. Negative first tier testing rules out hemolysis. Most tests for anemia involve a blood sample that is drawn from your arm using a needle or from a prick of the finger. RBC: Two 500-μL lavender-top Microtainer™ tubes filled to at least 50% of tube capacity. Collect. <>
Found inside – Page 9-40Test interpretation: poststimulation cortisol >22 ug/dL is consistent with HAC for most labs; <15 ug/dL is not consistent; ... for hemolysis and lipemia; promptly spin and separate serum/plasma before refrigeration Baseline blood sample ... Draw a picture of your slide agglutination results. For each culture, describe the colony morphology (macroscopic appearance of the growth) on blood agar plates. Devices that analyze serum specimens (biochemistry tests) monitor three aspects of serum: lipemia, icterus index and hemolysis index. 3 0 obj
After they die they break down and are removed from the circulation by the spleen. from
Tested Stain Reaction Urease Citrate Motility Indole ODC LDC Agar XLT-4 Green Glucose Sucrose Mannitol Lactose Glucose
Identification of Unknown =
Identification of Unknown =
EXERCISE 7
LABORATORY RESULTS WORKSHEETS (3 pages)
Name Lab Coordinator
A. Broth Tube MIC and Subculture MBC
Complete the following table:
Final Growth of S. aureus: Growth of Unknown: Concentration On TSA On TSA
of Tetracycline In Tube Subculture In Tube Subculture
Tube 1
Tube 2
Tube 3
Tube 4
Tube 5
Tube 6
Tube 7
Tube 8
What is the MIC of tetracycline for this isolate of S. aureus? Note that the percentage of hemolysis on sucrose hemolysis test can vary based on the temperature . Laboratory evaluation of a suspected hemolytic reaction can follow a hierarchic approach. • Identify limitations of sensitivity testing. Patients usually have decreased hemoglobin concentration, hematocrit, and red blood cell count, but some can have compensated disorders, and symptoms such as reticulocytosis, pigmented gallstones, and decreased haptoglobin are factors that raise clinical suspicion. EXERCISE 2
LABORATORY RESULTS WORKSHEET
Name Lab Coordinator
Organism Colony Gram
Tested Morphology Stain Hemolysis Coagulase Catalase Oxidase Fibrinolysin
Identification of Unknown = Identification of Unknown =
Identification of Unknown = Identification of Unknown = EXERCISE 3
LABORATORY RESULTS WORKSHEETS (2 pages)
Name Lab Coordinator
1. Frequently, clinical specimens are contaminated due to hemolysis with rates up to 20% in specimens collected from patients in the Emergency Department [86].Presence of hemolysis can cause either positive or negative . Direct 2 0 obj
Anemia can be detected on routine blood work, or tests for anemia may be ordered after a person has symptoms consistent with anemia. Name Lab Coordinator
5. Author Contributions:All authors confirmed they have contributed to the intellectual content of this paper and have met the following 4 requirements: (a) significant contributions to the conception and design, acquisition of data, or analysis and interpretation of data; (b) drafting or revising the article for intellectual content; (c) final . Hemolytic anemia is a clinically heterogeneous disorder with equally heterogeneous presentations. Hemolytic Found inside – Page 1107The detection of intravascular hemolysis in horse : TC McGuire , JB Henson . Fed Proc 28 : 425 , 69. ... Al - 07474-03 ( BM ) LOZANO , EDGARDO A , Veterinary Res Lab , Montana State Univ , Bozeman , Mont . 59715. absolute reticulocyte Hemolytic anemia is defined by the premature destruction of red blood cells, and can be chronic or life-threatening. There are numerous causes of hemolytic anemia, including inherited and acquired conditions, acute and chronic processes, and mild to potentially life-threatening severity.
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